Background experimental information:
1. The damage of mitochondrial DNA as a marker of oxidative stress, which has been demonstrated in a skin cell model. Anthocyanins (or their metabolites) may able to reduce mitochondrial DNA damage as a marker of oxidative stress (as well as general oxidative stress in the cell).
2. The previously evaluated HDFn skin fibroblast model is treated with hydrogen peroxide stressing.
3. Incubate the skin cells with different concentrations of Acai extract, individual anthocyanins and/or metabolite PCA for two-time points (e.g. 6h and 24h) and subsequently stress with 250uM hydrogen peroxide for 1h.
4. Isolating DNA and performing real-time qPCR to evaluate a potential reduction of mtDNA damage induced by H202.
5. For the general cell stress measurement, using DCF-DA fluorescent probe.
6. MitoSox could be used to measure also mitochondria specific ROS production.
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